你如果用买来的公司的MARKER瓶子上面会有,我用的那个公司是0.5UL,所以我们一般都会把它稀释50倍后再用。。。
无所谓浓度,各条ladder跑出来就成。定量的时候可以考虑“浓度”的问题,这个就看各公司ladder浓度的说明了。
买的marke买来的可以直接用的吧
一般5微升
In my experience, 100 ng per band (or DNA fragment) is necessary to make it visible with UV when the DNA bind EB. It is also variable for the size of the DNA fragment. Therefore, you can calculate how much you would like to include in your gel.
Good luck!