大肠杆菌用伊红美兰鉴别?

2025-03-15 22:28:20
推荐回答(2个)
回答1:

可以啊,步骤如下:
①制作伊红美蓝培养基,趁热注入培养皿中,凝成平板,待用。

②用灭过菌的锥形瓶盛取河水或沟水,按1:10稀释。

③取0.1毫升稀释液接种于伊红美蓝培养基上。用平板划线分离法进行分离。

④将划线后的培养皿倒置37℃温箱中培养18~24小时。培养基中会出现大肠杆菌菌落,菌落中心呈暗蓝黑色,发金属光泽。

⑤将菌落接种于斜面培养基上(由营养琼脂培养基制成)。

大肠杆菌的分离鉴定�
分离菌接种麦康凯培养基37 ℃ 24 h后,呈现边缘整齐或波状、稍突起,表面光滑湿润,直径1 mm~2 mm、粉红色或深红色的圆形菌落,在伊红美蓝琼脂上呈紫黑色带金属光泽的圆形菌落,在普通琼脂平板上呈灰白色,直径1.5 mm~2 mm的圆形菌 FL) 落;细菌涂片染色检查为革兰氏阴性、大小一致的杆菌。�
3株地方分离株分别接种到三糖铁培养基上,经37 ℃ 72 h培养,产酸、产气,不产生硫化氢。生化特征为:分解葡萄糖、乳糖、甘露醇,产酸产气;分解阿拉伯糖;吲哚试验、甲基红试验呈阳性反应;尿素酶、明胶液化、柠檬酸盐利用试验,VP试验等呈阴性反应。�
对分离的3株地方菌株分别用中国兽药监察所提供的大肠杆菌“O”抗原定型血清进行分型。其中2株血清型分别是O2、O35,另1株未能分型。

回答2:

在含有伊红美兰的培养基上画线培养,得到蓝紫色有金属光泽的菌落,则为大肠杆菌菌落~~

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