把已知序列DNA作为模板，通过引物设计软件如Primer5.0，可以自行设计引物，具体怎么设计，你可以到网上下下来自学。
是太现实了。。。高考的都是扯淡了~
1. If you have primer design software, you can use it.
2. if you do not, select some region with average GC contact, no repeat sequence, usually is OK. The length can be 18 to 25 nucleotides.
3. I doubt the national college entrance test will have such kind of question.
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